【英文标准名称】:StandardGuideforConductingToxicityTestswithBioluminescentDinoflagellates
【原文标准名称】:生物荧光双鞭甲藻毒性试验的标准指南
【标准号】:ASTME1924-1997(2004)
【标准状态】:现行
【国别】:
【发布日期】:1997
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E47.01
【标准类型】:(Guide)
【标准水平】:()
【中文主题词】:抑制;毒性试验;暗相;双鞭甲藻;光输出;生物荧光
【英文主题词】:bioluminescence;darkphase;dinoflagellate;GonyaulaxPloyedra;inhibition;lightoutput;Pyrocystislanula;toxicitytest
【摘要】:Protectionofaquaticspeciesrequirespreventionofunacceptableeffectsonpopulationsinnaturalhabitats.Toxicitytestsareconductedtoprovidedatatopredictwhatchangesinviablenumbersofindividualspeciesmightresultfromsimilarexposureinthenaturalhabit.Informationmightalsobeobtainedontheeffectsofthematerialonthehealthofotherspecies.Bioluminescentdinoflagellatesrepresentanimportanteucaryoticgroupwhicharewidelydistributedintheoceanicenvironment.1.1Thisguidecoverstwodistinctprocedures,basedonsimilarprinciples,forobtainingdataconcerningtheadverseeffectsofatestmaterial(addedtodilutionwater)onoceanicbioluminescentdinoflagellates.1.1.1Theendpointforbothproceduresisbasedonameasurablereductionorinhibitioninlightoutputfromthedinoflagellates.Bothproceduresaresimilarinthatwhenbioluminescentdinoflagellatesareexposedtotoxicants,ameasurablereductioninbioluminescenceisobservedfromtheircellsfollowingmechanicalstimulationwhencomparedtocontrolcells.Inthefirstprocedure,cellsofthebioluminescentdinoflagellateGonyaulaxpolyedracanbetestedoverarangeofuptosevendaysofexposure(orlonger)toatoxicant.Thesecondprocedureusesanotherspecies,Pyrocystislunula,fora4htest.1.2Bothprocedurescanmeasurethetoxiceffectsofmanychemicals,variousmarineandfreshwatereffluents,antifoulingcoatings,leachates,andsedimentstobioluminescentdinoflagellates(1-5).Compoundswithlowwatersolubilitysuchaslargeorganicmoleculesmaybesolubilizedwithmethanol,ethanol,andacetonesolventsfortesting(4)(seeGuideE729).1.3AnIC50inlightoutput(bioluminescence)istherecommendedendpoint(1).However,percentinhibitionofbioluminescenceisanappropriateendpointinsomecases(5).1.4Othermodificationsoftheseproceduresmightbejustifiedbyspecialneedsorcircumstances.Althoughusingappropriateproceduresismoreimportantthanfollowingprescribedprocedures,resultsoftestsconductedusingunusualproceduresarenotlikelytobecomparabletoresultsofothertests.Comparisonofresultsobtainedusingmodifiedandunmodifiedversionsoftheseproceduresmightprovideusefulinformationconcerningnewconceptsandproceduresforconductingacuteandchronictests.1.5ThevaluesstatedinSIunitsaretoberegardedasthestandard.1.6Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:Z04
【国际标准分类号】:11_120_01
【页数】:12P.;A4
【正文语种】: